High pure rna isolation kit roche manual

The High Pure Plasmid Isolation Kit components should be stored at room temperature (+15 to +25°C). Kit componen ts are guaranteed to be stable until the expiration date printed on the label. After adding RNase A, store the Suspension Buffer at +2 to +8°C, where it will be stable for 6 months. The High Pure FFPET RNA Isolation Kit uses a fast and optimized technology for the isolation and purification of total RNA from formalin-fixed, paraffin-embedded tissue research samples. The quality of RNA from tissue samples is suitable for the following downstream applications: • RT-PCR • Gene Expression Array • Next Generation Sequencing.  · The High Pure FFPET RNA Isolation Kit is designed for the isolation and purification of total RNA from 5 to 10 μm thick sections from formalin-fixed, paraffin-embedded tissue. Macrodissected samples or biopsy material can also be used.

Figure 1: RT-PCR analysis of MS2 RNA isolated with the High Pure Viral RNA Kit. Serial dilutions of purified MS2 RNA were applied to High Pure filter tubes, processed according to the kit protocol, and eluted in a total volume of 50 µL. An aliquot ( µL) of each eluate was analyzed by two-step RT-PCR, using primers that produced a bp. The High Pure Viral Nucleic Acid Kit isolates highly purified viral nucleic acids (DNA and RNA) from mammalian plasma, serum, or whole blood. The purified viral nucleic acid may be used directly as templates for standard or long-template PCR. The High Pure Plasmid Isolation Kit components should be stored at room temperature (+15 to +25°C). Kit componen ts are guaranteed to be stable until the expiration date printed on the label. After adding RNase A, store the Suspension Buffer at +2 to +8°C, where it will be stable for 6 months.

27 ก.พ. There are several methods for miRNA extraction using manual or automated We used the conventional HighPure miRNA isolation kit (Roche. High Pure FFPET RNA Isolation Kit compatible with manual or automated nucleic acid extraction methods such as the. MagNA Pure 96 System. Quantification of human immunodeficiency virus type 1 (HIV-1) RNA viral load in by manual High Pure Viral Nucleic Acid Isolation kit (Roche Diagnostics.